The present study was conducted to ascertain the seroprevalence of bluetongue in cattle and buffalo population in Punjab, India. Total 92 serum samples were collected from female bovines belonging to 3 different agroclimatic zones of Punjab and subjected to cELISA test for the detection of anti-bluetongue antibodies. The overall apparent seroprevalence of bluetongue was 8.7%, while true serological prevalence was calculated 7.8%. There is no clinical report on bluetongue in small and large ruminants in Punjab. Though, a very high seroprevalence (53.0%) was reported in sheep (58.6%) and goat (50.6%) population of the state in a previous study. The prevalent serotypes of BTV circulating in this region are needed to identify in further study.